Co-expression of Interleukin-17A molecular adjuvant and prophylactic Helicobacter pylori genetic vaccine could cause sterile immunity in Treg suppressed mice.
Increased clinical significance of Helicobacter pylori (H. pylori) in human gastric cancer has led global efforts to combat these pathogens. Recent studies have confirmed the importance of some cytokines such as interleukin-18 (IL-18), Interleukin-8 (IL-8), interleukin-17A (IL-17A) and Interleukin-22 (IL-22) in the pathogenesis of the so-called bacteria.
This study was designed to compare the effects of Type 1T helper (Th1), 2T type helper (Th2) cell, Regulatory T cells (Treg) and T helper 17 (Th17) modulatory effect on the efficacy of H. pylori vaccine designed to combine multiple adjuvant molecules in Treg Treg competent and suppressed groups. A bicistronic vector used for simultaneous expression of inflammatory proteins Outer codon-optimized (OipA) gene modified mice and IL-18, IL-17A, IL-22 and Foxp3 (forkhead box P3) cytokine of four tapes. Immunization groups of mice were carried out by using the resulting plasmid intradermally.
Specific IgG1 and IgG2 and IgA titers of antibodies produced in mice was confirmed by enzyme-linked immunosorbent assay (ELISA) in serum and gut obtained four weeks after the last immunization. Once stimulated with a mixture of both anti-CD28 mAb and H. pylori lysate, a single frequency Interferon-gamma (IFN-γ), IL-17 single and dual IFN-γ / IL-17-secreting T-cells are documented using a dual-color FluoroSpot , Kinetics of Th1, Th2 and Th17 in the immunized animals was determined by the relative quantification of IL-17A, IL-22, IFN-γ, IL-8, IL-2 and IL-4 mRNA specific.
Four weeks after bacterial challenge, the number of colonies in the stomach quantitative isolated and homogeneous used to assess the level of protective immunity among all groups. Immunological test results showed that the highest cell-mediated immune cytokine IL-17 is produced in the receiver group in which Treg responses previously suppressed by Foxp3 administration as an immunogen. In addition, powerful cleaning of Helicobacter pylori infection seen in this group as well.
Co-expression of Interleukin-17A molecular adjuvant and prophylactic Helicobacter pylori genetic vaccine could cause sterile immunity in Treg suppressed mice.
The relationship between interleukin 6 receptor genetic variants and risk of functional and functional results of a large artery atherosclerotic stroke.
To investigate whether Asp358Ala interleukin 6 receptor (IL6R) associated with risk and large artery atherosclerosis (LAA) Han Chinese.A stroke in a prospective cohort study conducted in 768 patients with LAA stroke and non-stroke control 686. Genotypes of Asp358Ala polymorphisms were determined using SNPscan technology. The relationship between genotype and risk of stroke LAA were analyzed by logistic regression model.CC genotypes (P <0.001) and AC genotype (P = 0.023) lower the risk of stroke LAA compared with the AA genotype.
Multivariate logistic regression analysis showed that the CC genotype was significantly associated with risk of stroke LAA (P = 0.002). In a subgroup analysis, polymorphism Asp358Ala were significantly associated with the risk of LAA stroke in the additive model, the dominant model and model of recessive (P = 0.009, P = 0.017, P = 0.012, respectively) for men but not for women.
IL-2 Interleukin-2 Human Recombinant Protein, His Tag
Description: Interleukin-2 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 133 amino acids fragment (21-153) having a molecular weight of 20kDa and fused with a 4.5kDa amino-terminal hexahistidine tag. _x000D_ The IL-2 His is purified by proprietary chromatographic techniques._x000D_
Description: Interleukin-7 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids and having a molecular mass of 17.4 kDa. ;The IL-7 is purified by proprietary chromatographic techniques.
Description: Interleukin-10 Human Recombinant produced in E.coli is a single non-glycosylated polypeptide chains containing 161 amino acids each and having a molecular mass of 18.6kDa.;The IL-10 is purified by proprietary chromatographic techniques.
Description: A competitive ELISA for quantitative measurement of Rat Interleukin 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Interleukin 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Interleukin 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Pancreas PrimaCell 2: Pancreatic Epithelial Cells
Description: Interleukin-4 Human Recombinant produced in CHO is a single, non-glycosylated polypeptide chain containing 129 amino acids and having a molecular mass of 14 kDa, the glycosilation of IL-4 migrates as 18 kDa on SDS-PAGE.;The IL-4 is purified by proprietary chromatographic techniques.
Description: Interleukin-6 Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 193 amino acids (25-211a.a.) and having a molecular mass of 22.5kDa (Molecular size on SDS-PAGE will appear at approximately 18-28kDa). IL6 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Description: Interleukin-3 Human Recombinant produced in insect cells is a single, glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15000 Dalton. ;The IL-3 CSF is fused to a C-terminal His-tag (6x His) and purified by proprietary chromatographic techniques.
Description: IL-9 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (19-144 a.a.) including an 18 aa signal peptide (1-18 aa) and fused to a 6 aa His Tag at C-terminus containing a total of 150 amino acids and having a molecular mass of 14.5kDa.;IL9 shows multiple bands between 18-28kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
IL15 Human, Interleukin-15 Human Recombinant Protein, HEK
Description: Interleukin-15 Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight of 12.8kDa due to glycosylation.;The IL15 is purified by proprietary chromatographic techniques.
Description: Pegylated Interleukin-22 Mouse Recombinant produced in E.Coli is a single, non-glycosylated homodimeric polypeptide chain containing 147 amino acids and an aditional Ala amino acid at N-terminus having a molecular mass of 36 kDa as determioned by mass spectometry. However due to enlarged hydrodymanic volume it runs on the SDS-PAGE as a 50 kDa protein and in gel-filtration on Superdex 200 as over 200 kDa protein._x000D_ The Murine IL-22 is Mono-pegylated (with 20 kDa PEG) purified by proprietary chromatographic techniques. _x000D_
IL17B Human, Interleukin-17B Human Recombinant Protein, Sf9
Description: IL17B Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 169 amino acids (21-180a.a.) and having a molecular mass of 19.2kDa (Molecular size on SDS-PAGE will appear at approximately 18-28kDa). IL17B is expressed with a 6 amino acid His-tag at C-Terminus and purified by proprietary chromatographic techniques.
Description: Interleukin-1 alpha Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight of 18kDa due to glycosylation.;The IL1A is purified by proprietary chromatographic techniques.
IL-12-p40 Interleukin-12 p40 Human Recombinant Protein
Description: Interleukin-12 p40 His Human Recombinant produced in E.Coli is single, a non-glycosylated, polypeptide chain containing 306 amino acids fragment (23-328) with an amino-terminal hexahistidine tag. ;The IL-12 p40 His is purified by proprietary chromatographic techniques.
IL-13-Variant Interleukin-13 Variant Human Recombinant Protein
Description: Interleukin-13 Variant Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 114 amino acids, with a substitution of Q for R at position 112 compared with the wild type IL-13, having a molecular mass of 12.5 kDa. ;The IL-13 Variant is purified by proprietary chromatographic techniques.
IL-16 Interleukin-16 Human Recombinant Protein, His Tag
Description: Interleukin-16 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain (502-631 a.a) containing 150 amino acids and having a molecular mass of 15.5kDa. The IL-16 is fused to a 20 a.a His-Tag at N-Terminus.;The IL-16 is purified by proprietary chromatographic techniques.
IL-17 Interleukin-17 Human Recombinant Protein, His Tag
Description: Interleukin-17A Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 132 amino acids fragment (24-155) having a molecular weight of 19.62kDa and fused with a 4.5kDa amino-terminal hexahistidine tag. ;The IL-17A His is purified by proprietary chromatographic techniques.
IL-29 Interleukin-29 Human Recombinant Protein, His Tag
Description: IL 29 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 206 amino acids (20-200 a.a) and having a molecular mass of 22.7kDa.IL 29 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
IL-28A Interleukin-28A Human Recombinant Protein, His Tag
Description: IL 28A Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 198 amino acids (26-200 a.a.) and having a molecular mass of 22.1kDa. IL 28A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
IL36G Interleukin-36 Gamma Human Recombinant Protein, His Tag
Description: IL36G Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 192 amino acids (1-169) and having a molecular mass of 21.1kDa.
Description: Quantitativesandwich ELISA kit for measuring Rat Interleukin 2, IL-2 in samples from serum, urine, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Rat Interleukin 2, IL-2 in samples from serum, urine, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Interleukin-2 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 17kDa. ;The IL-2 is purified by proprietary chromatographic techniques.
Further regression analysis showed that compared to those with the AA genotype and obesity, those with the CC genotype and non-obese were less likely to suffer a stroke LAA (P = 0.003). For the male participants, the association is still there (additive model, P = 0.022). After 3 months of follow-up, patients with the C allele had a good functional prognosis compared to patients with the A allele (P = 0.009) .the Asp358Ala study showed that polymorphism may be associated with susceptibility to the development and outcome of stroke in Han Chines LAA.
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